![TP05 Primer design and melting temperature calculations for PCR primers - Primer design and melting - Studocu TP05 Primer design and melting temperature calculations for PCR primers - Primer design and melting - Studocu](https://d20ohkaloyme4g.cloudfront.net/img/document_thumbnails/48b5bfa14ef28a2d6166050c2f380fad/thumb_1200_1697.png)
TP05 Primer design and melting temperature calculations for PCR primers - Primer design and melting - Studocu
![SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule." This equation is: Tm = 2(A + T) + 4(G + C) Where SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule." This equation is: Tm = 2(A + T) + 4(G + C) Where](https://cdn.numerade.com/ask_images/e6d23eaac0be47f7af19c3bc2e728e3d.jpg)
SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule." This equation is: Tm = 2(A + T) + 4(G + C) Where
![SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (% SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%](https://cdn.numerade.com/ask_previews/6f0c47ee-38d7-4778-b4d8-d117b7d1affe_large.jpg)